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ifn γ evaluation by elisa  (Elabscience Biotechnology)


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    Elabscience Biotechnology ifn γ evaluation by elisa
    Ifn γ Evaluation By Elisa, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 181 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ifn+%CE%B3+evaluation+by+elisa/Mouse+IFN-%CE%B3+(Interferon+Gamma)+ELISA+Kit/pm41565700-427-40-47
    Average 96 stars, based on 181 article reviews
    ifn γ evaluation by elisa - by Bioz Stars, 2026-10
    96/100 stars

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    Enzyme-linked Immunosorbent Assay:

    Article Title: In situ self-assembled cell reservoir hydrogel for maneuvering multistage radioimmunotherapy
    Article Snippet: The splenocytes were extracted and processed to remove RBC by lysis buffer (Beyotime, Shanghai, China). .. The obtained cells were seeded in a 96-well plate and subjected to free IL-12 or IL@RBC at pre-designated concentrations at 37 °C for 48 h. The culture supernatants were respectively collected for IFN-γ evaluation by ELISA (Mouse IFN-γ ELISA, Elabscience), and normalized by the whole protein in the samples. ..

    Article Title: In situ self-assembled cell reservoir hydrogel for maneuvering multistage radioimmunotherapy.
    Article Snippet: The splenocytes were extracted and processed to remove RBC by lysis buffer (Beyotime, Shanghai, China). .. The obtained cells were seeded in a 96-well plate and subjected to AR TI CL E IN P RE SS free IL-12 or IL@RBC at pre-designated concentrations at 37 °C for 48 h. The culture supernatants were respectively collected for IFN-γ evaluation by ELISA (Mouse IFN-γ ELISA, Elabscience), and normalized by the whole protein in the samples. ..



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    ifn γ evaluation by elisa  (Elabscience Biotechnology)
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    Deficiency of miR-302 accelerates lipopolysaccharide (LPS)-induced vascular inflammation. (A) The survival rate of 8-weeks miR-302 +/− mice (302 +/− ) and WT mice after LPS injection (25 mg/kg), n = 20. (B) The expression of ICAM-1 in aorta from WT and miR-302 +/− mice was detected by immunofluorescence, n = 5, scale bar, 10 μm, asterisk indicates the lumen of aorta. (C) Immunohistochemical staining of aorta VCAM-1 from WT and miR-302 +/− mice injected with LPS, n = 6, scale bar, 100 μm. (D) Immunofluorescence staining of F4/80 represents the infiltration of macrophages (red) in the aorta, nuclei were counterstained with DAPI (blue), n = 5, scale bar, 50 μm. The level of inflammatory factors <t>E-selectin</t> (E) , IFN-γ (F) , IL-6 (G) , and MCP-1 (H) in serum from miR-302 +/− mice and WT mice, n = 6. The inflammatory status of lung (I) and liver (J) stained by HE staining, n = 6. Scale bar, 100 μm (* p < 0.05; ** p < 0.01; *** p < 0.001 vs. WT + LPS).
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    Deficiency of miR-302 accelerates lipopolysaccharide (LPS)-induced vascular inflammation. (A) The survival rate of 8-weeks miR-302 +/− mice (302 +/− ) and WT mice after LPS injection (25 mg/kg), n = 20. (B) The expression of ICAM-1 in aorta from WT and miR-302 +/− mice was detected by immunofluorescence, n = 5, scale bar, 10 μm, asterisk indicates the lumen of aorta. (C) Immunohistochemical staining of aorta VCAM-1 from WT and miR-302 +/− mice injected with LPS, n = 6, scale bar, 100 μm. (D) Immunofluorescence staining of F4/80 represents the infiltration of macrophages (red) in the aorta, nuclei were counterstained with DAPI (blue), n = 5, scale bar, 50 μm. The level of inflammatory factors E-selectin (E) , IFN-γ (F) , IL-6 (G) , and MCP-1 (H) in serum from miR-302 +/− mice and WT mice, n = 6. The inflammatory status of lung (I) and liver (J) stained by HE staining, n = 6. Scale bar, 100 μm (* p < 0.05; ** p < 0.01; *** p < 0.001 vs. WT + LPS).

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: MiR-302a Limits Vascular Inflammation by Suppressing Nuclear Factor-κ B Pathway in Endothelial Cells

    doi: 10.3389/fcell.2021.682574

    Figure Lengend Snippet: Deficiency of miR-302 accelerates lipopolysaccharide (LPS)-induced vascular inflammation. (A) The survival rate of 8-weeks miR-302 +/− mice (302 +/− ) and WT mice after LPS injection (25 mg/kg), n = 20. (B) The expression of ICAM-1 in aorta from WT and miR-302 +/− mice was detected by immunofluorescence, n = 5, scale bar, 10 μm, asterisk indicates the lumen of aorta. (C) Immunohistochemical staining of aorta VCAM-1 from WT and miR-302 +/− mice injected with LPS, n = 6, scale bar, 100 μm. (D) Immunofluorescence staining of F4/80 represents the infiltration of macrophages (red) in the aorta, nuclei were counterstained with DAPI (blue), n = 5, scale bar, 50 μm. The level of inflammatory factors E-selectin (E) , IFN-γ (F) , IL-6 (G) , and MCP-1 (H) in serum from miR-302 +/− mice and WT mice, n = 6. The inflammatory status of lung (I) and liver (J) stained by HE staining, n = 6. Scale bar, 100 μm (* p < 0.05; ** p < 0.01; *** p < 0.001 vs. WT + LPS).

    Article Snippet: ELISA kit to evaluate ICAM-1, VCAM-1, IL-6, MCP-1, IFN-γ, and E-selectin were from Boster Biological Engineering Co (Wuhan, China).

    Techniques: Injection, Expressing, Immunofluorescence, Immunohistochemical staining, Staining

    MiR-302a-mimics weakens LPS-induced inflammation of mice. (A) The survival rate of 8 weeks’ WT mice injected with miR-302a mimics (302a-m) or negative control mimics (NC-m). WT mice were injected with LPS (25 mg/kg), n = 20. (B) Immunofluorescence of ICAM-1 in aorta from mice injected with miR-302a mimics or negative control mimics. n = 6, scale bar, 10 μm, asterisk indicated the lumen of aorta. (C) Immunohistochemical staining of VCAM-1 of aorta from mice injected with mimics. n = 5, scale bar, 100 μm. (D) Immunofluorescence staining of F4/80 represents the infiltration of macrophages (red) in the aorta, nuclei were counterstained with DAPI (blue), n = 6, scale bar, 50 μm. The inflammatory factors of E-selectin (E) , IFN-γ (F) , IL-6 (G) , and MCP-1 (H) in serum of mice ( n = 6). The inflammatory status in lung (I) and liver (J) stained by HE, n = 6, scale bar, 100 μm (* p < 0.05; ** p < 0.01; *** p < 0.001 vs. NC-m + LPS).

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: MiR-302a Limits Vascular Inflammation by Suppressing Nuclear Factor-κ B Pathway in Endothelial Cells

    doi: 10.3389/fcell.2021.682574

    Figure Lengend Snippet: MiR-302a-mimics weakens LPS-induced inflammation of mice. (A) The survival rate of 8 weeks’ WT mice injected with miR-302a mimics (302a-m) or negative control mimics (NC-m). WT mice were injected with LPS (25 mg/kg), n = 20. (B) Immunofluorescence of ICAM-1 in aorta from mice injected with miR-302a mimics or negative control mimics. n = 6, scale bar, 10 μm, asterisk indicated the lumen of aorta. (C) Immunohistochemical staining of VCAM-1 of aorta from mice injected with mimics. n = 5, scale bar, 100 μm. (D) Immunofluorescence staining of F4/80 represents the infiltration of macrophages (red) in the aorta, nuclei were counterstained with DAPI (blue), n = 6, scale bar, 50 μm. The inflammatory factors of E-selectin (E) , IFN-γ (F) , IL-6 (G) , and MCP-1 (H) in serum of mice ( n = 6). The inflammatory status in lung (I) and liver (J) stained by HE, n = 6, scale bar, 100 μm (* p < 0.05; ** p < 0.01; *** p < 0.001 vs. NC-m + LPS).

    Article Snippet: ELISA kit to evaluate ICAM-1, VCAM-1, IL-6, MCP-1, IFN-γ, and E-selectin were from Boster Biological Engineering Co (Wuhan, China).

    Techniques: Injection, Negative Control, Immunofluorescence, Immunohistochemical staining, Staining